MACS SmartStrainer, 30µM from Miltenyi Biotec

(1) Chlamydial YAP activation in host endocervical epithelial cells mediates pro-fibrotic paracrine stimulation of fibroblastsmSystemsDecember 21, 2023Liam Caven, Rey Carabeoeliminate residual trypsin, cell suspensions were filtered over 30-µm cell strainers (Miltenyi Biotec, 130-098-458) to eliminate clustered cells, washed twice in 1× phosphate-buffered saline (PBS) containing 0.04% bovine

(2) Differential requirements for CD4+ T cells in the efficacy of the anti-PD-1+LAG-3 and anti-PD-1+CTLA-4 combinations in melanoma flank and brain metastasis modelsJ Immunother CancerDecember 6, 2023Manali S Phadke, Jiannong Li, Zhihua Chen, Paulo C Rodriguez, Jessica K Mandula, Lilit Karapetyan, Peter A Forsyth, Y Ann Chen, Keiran S M Smalleydissociation kit (cat. #130-095-929; Miltenyi Biotec). Cells were strained through MACS strainer (cat. # 130-098-458; Miltenyi Biotec). The cell count and viability were analyzed by staining the cells with AO/PI stain

(3) Gut microbiota promotes enteroendocrine cell maturation and mitochondrial functionbioRxivNovember 9, 2023Alfahdah Alsudayri, Shane Perelman, Annika Chura, Melissa Brewer, Madelyn McDevitt, Catherine Drerup, Lihua YeHI-FBS, 10 mM HEPES and 2 mM EDTA], samples were filtered through 30 μm cell strainers (Miltenyi Biotec, 130-098-458). Samples were then centrifuged at 1800 rcf for 15 minutes at room temperature. The supernatant was

(4) Integrative analysis of single-cell RNA-seq and ATAC-seq reveals heterogeneity of induced pluripotent stem cell-derived hepatic organoidsiScienceSeptember 15, 2023Jong-Hwan Kim, Seon Ju Mun, Jeong-Hwan Kim, Myung Jin Son, Seon-Young KimFisher; 12605-010), and the aggregated cells were removed using MACS Smart Strainers (Miltenyi Biotec; 130-098-458). Cell viability was measured with Countess II FL (Thermo Fisher Scientific), and single cells with

(5) Protocol to dissociate and isolate wide-diversity single cells by density gradient centrifugation from human hepatoblastoma tissueSTAR ProtocolsSeptember 1, 2023Xianchao Zhou, Mei Meng, Yifan Wu, Rui Gao, Yuhua Shan, Song Gu, Jian HeBS-10-TRS SmartStrainer 70 μm,Miltenyi Biotec,Cat# 130-098-462 SmartStrainer 30 μm,Miltenyi Biotec,Cat# 130-098-458 Invitrogen Countess Cell Counting Chamber Slides,Thermo Fisher,Cat# C10228 Qubit Assay Tubes,Thermo Fisher

(6) Multi-omics analysis identifies IgG2b class-switching with ALCAM-CD6 co-stimulation in joint-draining lymph nodes during advanced inflammatory-erosive arthritisFrontiers in ImmunologyAugust 25, 2023H. Mark Kenney, Javier Rangel-Moreno, Yue Peng, Kiana L. Chen, Jennifer Bruno, Abdul Embong, Elizabeth Pritchett, Jeffrey I. Fox, Enrique Becerril-Villanueva, Armando Gamboa-Domínguez, et al.the tissue in 3mL of 20% FBS in PBS using a scalpel. A 30μm MACS SmartStrainer (Miltenyi Biotec, Cat# 130-098-458) was pre-wet with 3 mL of 20% FBS, the cell suspensions were passed through, and 20% FBS was added until

(7) Calcitonin Related Polypeptide Alpha Mediates Oral Cancer PainCellsJune 21, 2023Tu NH, Inoue K, Lewis PK, Khan A, Hwang JH, Chokshi V, Dabovic BB, Selvaraj S, Bhattacharya A, Dubeykovskaya Z, et al.5 min. The cell pellets were resuspended in 1 mL DMEM, filtered through 30 µm cell strainers (Cat# 130-098-458, Miltenyi, Gaithersburg, MD, USA) into 2 mL Eppendorf tubes and centrifuged at 150 rcf for 3 min to

(8) Adoptive T cell transfer and host antigen-presenting cell recruitment with cryogel scaffolds promotes long-term protection against solid tumorsNature CommunicationsJune 15, 2023Kwasi Adu-Berchie, Joshua M. Brockman, Yutong Liu, Tania W. To, David K. Y. Zhang, Alexander J. Najibi, Yoav Binenbaum, Alexander Stafford, Nikolaos Dimitrakakis, Miguel C. Sobral, et al.using MACS buffer: DPBS with 0.5% BSA and 2 mM EDTA, and filtered through a 30-µm strainer (Miltenyi #130-098-458). Lymph nodes Brachial, axillary, and inguinal lymph nodes were harvested and pooled before digestion.

(9) Pathogen-specific innate immune response patterns are distinctly affected by genetic diversityNature CommunicationsJune 5, 2023Antje Häder, Sascha Schäuble, Jan Gehlen, Nadja Thielemann, Benedikt C. Buerfent, Vitalia Schüller, Timo Hess, Thomas Wolf, Julia Schröder, Michael Weber, et al.double-distilled H2O and separated from mycelia by using MACS SmartStrainers (30 µm, Miltenyi Biotec Cat# 130-098-458). For induction of germ tubes, 4 × 106 conidia/ml were incubated for 5 h to 6 h in RPMI1640 with L-glutamine

(10) Integrative Single-Cell Transcriptomics and Epigenomics Mapping of the Fetal Retina Developmental DynamicsAdvanced science (Weinheim, Baden-Wurttemberg, Germany)June 1, 2023Li R, Liu J, Yi P, Yang X, Chen J, Zhao C, Liao X, Wang X, Xu Z, Lu H, et al.of 2 mg mL−1 papain (LS003119, Worthington) for ≈20 min at 37 °C. Using a 30µm MACS Smart Strainer (130‐098‐458, Miltenyi), the dissociated fetal eyeball tissue was filtered to guarantee single‐cell suspensions.

(11) The First Transcriptomic Atlas of the Adult Lacrimal Gland Reveals Epithelial Complexity and Identifies Novel Progenitor Cells in MiceCellsMay 21, 2023Delcroix V, Mauduit O, Lee HS, Ivanova A, Umazume T, Knox SM, de Paiva CS, Dartt DA, Makarenkova HP.supplemented with 0.5% BSA and 10 mM HEPES, the cells were passed through a pre-wet 30-µm mesh cell strainer (#130-098-458, Miltenyi Biotec, Bergisch Gladbach, Germany). After centrifugation (400× g, 5 min, 4 °C), the dead

(12) An ERK-dependent molecular switch antagonizes fibrosis and promotes regeneration in spiny mice ( Acomys )Science AdvancesMay 1, 2023Antonio Tomasso, Tim Koopmans, Philip Lijnzaad, Kerstin Bartscherer, Ashley W. SeifertThe supernatant containing cells was filtered through a 30-μm MACS SmartStrainer (Miltenyi Biotec, 130-098-458). The remaining tissue was ground in a fresh enzyme mix for eight additional minutes, passed through

(13) A combinatorial panel for flow cytometry‐based isolation of enteric nervous system cells from human intestineEMBO reportsApril 5, 2023Jonathan D Windster, Andrea Sacchetti, Gerben J Schaaf, Eric MJ Bindels, Robert MW Hofstra, Rene MH Wijnen, Cornelius EJ Sloots, Maria M Alveswas removed with a wet wipe and discarded. The suspension was filtered through a 70 μm cell strainer (130‐098‐458, Miltenyi Biotec) and mixed 1:10 with HBSS buffer without Ca2+ Mg2+ (14175095, Life Technologies), and

(14) Cell-Specific Paired Interrogation of the Mouse Ovarian Epigenome and TranscriptomeJournal of Visualized ExperimentsMarch 13, 2023Sarah R. Ocañas, José V. V. Isola, Tatiana D. Saccon, Kevin D. Pham, Ana J. Chucair-Elliott, Augusto Schneider, Willard M. Freeman, Michael B. StoutScientific,50-255-956 Halt Protease Inhibitor cocktail (100X),Thermo Scientific,1861278 30 μm cell strainer,Miltenyi Biotec,130-098-458 M-280 Streptavidin Dynabeads,Invitrogen,11205D DynaMag-2 magnet,Invitrogen,12321D VWR Tube Rotator,Fisher

(15) N-acetylneuraminic acid links immune exhaustion and accelerated memory deficit in diet-induced obese Alzheimer’s disease mouse modelNature CommunicationsMarch 9, 2023Stefano Suzzi, Tommaso Croese, Adi Ravid, Or Gold, Abbe R. Clark, Sedi Medina, Daniel Kitsberg, Miriam Adam, Katherine A. Vernon, Eva Kohnert, et al.resuspended in 500 μL of wash buffer. Nuclei were filtered through a 30 μm MACS Smartstrainer (Miltenyi, 130-098-458) and counted using the LUNA-FL™ Dual Fluorescence Cell Counter (Logos Biosystems) after staining with

(16) Protocol for multi-modal single-cell RNA sequencing on M. tuberculosis-infected mouse lungsSTAR ProtocolsMarch 1, 2023Davide Pisu, David G. RussellGentleMacs Strainers,Miltenyi Biotech,Cat# 130-098-462 30μM GentleMacs Strainers,Miltenyi Biotech,Cat# 130-098-458 Collagenase IV,Worthington,Cat# LS004186 Cell strainers,VWR,Cat# 10199-656Cat# 10199-655 ACK lysis

(17) The lysophosphatidic acid-regulated signal transduction network in ovarian cancer cells and its role in actomyosin dynamics, cell migration and entosisTheranosticsJanuary 1, 2023Ojasalu K, Lieber S, Sokol AM, Nist A, Stiewe T, Bullwinkel I, Finkernagel F, Reinartz S, Müller-Brüsselbach S, Grosse R, et al.spheroids were obtained from ascites by consecutive filtration using 30 and 100 µm cell strainers (130-098-458 and 130-098-463, Miltenyi Biotech, Bergisch Gladbach, Germany). The cells were fixed with 4% formaldehyde,

(18) Transplanting rejuvenated blood stem cells extends lifespan of aged immunocompromised micenpj Regenerative MedicineDecember 29, 2022Sara Montserrat-Vazquez, Noelle J. Ali, Francesca Matteini, Javier Lozano, Tu Zhaowei, Eva Mejia-Ramirez, Gina Marka, Angelika Vollmer, Karin Soller, Mehmet Sacma, et al.Sigma Aldrich) and filtered through MACS Smartstrainers 70 µm (130-098-462, Miltenyi Biotec) and 30 µm (130-098-458, Miltenyi Biotec). The cell suspension was stained with lineage negative antibodies for 20 min at 4

(19) Single-cell transcriptome of the mouse retinal pigment epithelium in response to a low-dose of doxorubicinCommunications BiologyDecember 1, 2022Hyungwoo Lee, Ho-Yeon Lee, Jae-Byoung Chae, Chul-Woo Park, Chaekyu Kim, Ja-Hyoung Ryu, Jiwon Jang, Namshin Kim, Hyewon Chunghemocytometer. Single-cell RNA-Seq Suspended cells were filtered out using a 30 µm cell strainer (Miltenyi Biotech, cat no. 130-098-458) and washed two times with cold Ca2+- and Mg2+-free 0.04% BSA/PBS at 300 g for 5 min at 4 °C. After

(20) Single-cell chromatin accessibility landscape in kidney identifies additional cell-of-origin in heterogenous papillary renal cell carcinomaNature CommunicationsDecember 1, 2022Qi Wang et al.Tween-20 and 1% BSA) was then added and the resulting solution was filtered through a 30-µm cell strainer (130-098-458; Miltenyi Biotec). Nuclei were collected by centrifuging (500 g for 5 min at 4 °C) and resuspended in

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MACS SmartStrainers (30 µm), 50 filters (sterile, single-packed) from Miltenyi Biotec

MACS SmartStrainer, 30µM from Miltenyi Biotec

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Miltenyi Biotec

Miltenyi Biotec

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MACS SmartStrainer, 30µM from Miltenyi Biotec

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